Prediction of the Mechanism of Epimedin C Promoting Tunneling Nanotubes Production Based on Network Pharmacology
DOI:
https://doi.org/10.62767/jecacm604.0919Keywords:
Epimedin C, tunneling nanotubes, network pharmacologyAbstract
Background: To predict the mechanism of Tunneling nanotubes (TNTs) formation promoted by Epimedin C based on network pharmacology. Methods: The targets of Epimedin C were obtained through the PubChem, Pharm Mapper, Swiss Target Prediction and UniProt databases. Using the Genome Annotation Database Platform (GeneCards) database, the targets of TNTs were identified. The common targets were identified after intersection, and the STRING database was applied to construct a Protein-Protein Interaction (PPI) Network. The DAVID database was used for Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Results: Through network pharmacology, 11 key targets including ALB, IGF1, BMP2, ACHE, MMP2, LCN2, EGFR, CDK2, SRC, ANXA5, and BMP7 were screened. The GO analysis results involved signaling pathways such as the positive regulation of vascular smooth muscle cell proliferation, positive regulation of osteoblast differentiation, and epithelial to mesenchymal transition. The KEGG results were related to endocrine, bladder cancer, EGFR tyrosine kinase inhibitors of drug resistance, FoxO and other signaling pathways. Conclusions: Epimedin C may promote the generation of TNTs through the endocrine and EGFR tyrosine kinase inhibitor resistance signaling pathways. The findings of this study provide a theoretical basis for further investigation into the mechanism by which Epimedin C promotes TNTs generation.
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