Transcriptomic Analysis on the Key Genes and Functional Pathways of Ramulus Cinnamomi in Repressing the Proliferation of Lung Cancer in vitro

Shanyong Wu; Tao Wang; Jiayuan Wu; Hui Yuan; Jiazhen Xu; Hui Zhang; Ninghua Jiang

doi:10.62767/

Authors

Shanyong Wu Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314099 Jiaxing, Zhejiang, China Author
Tao Wang Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Di'An Diagnostics Group Co., Ltd., 310013 Hangzhou, Zhejiang, China Author
Jiayuan Wu Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314099 Jiaxing, Zhejiang, China Author
Hui Yuan Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Di'An Diagnostics Group Co., Ltd., 310013 Hangzhou, Zhejiang, China Author
Jiazhen Xu Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314099 Jiaxing, Zhejiang, China Author
Hui Zhang Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314099 Jiaxing, Zhejiang, China Author
Ninghua Jiang Department of Pharmacy, The Second Affiliated Hospital of Jiaxing University, 314099 Jiaxing, Zhejiang, China Author

DOI:

https://doi.org/10.62767/

Keywords:

Ramulus Cinnamomi, non-small cell lung carcinoma, transcriptomics, epidermal growth factor receptor, gefitinib

Abstract

Background: This study is engineered to investigate the key genes and functional pathways of Ramulus Cinnamomi (RC) in inhibiting the proliferation of lung cancer cell lines A549 and H1650 in vitro. Methods: The total RNA in A549 and H1650 cells was collected before and after treatment of RC, and transcriptomic sequencing was subsequently performed. Through the screening, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, Gene Ontology (GO) analysis, gene set enrichment analysis (GSEA) and protein-protein network interaction (PPI) analysis of differentially expressed genes (DEGs), the function enrichment and network construction of DEGs were carried out, and the selected key DEGs were finally verified by real-time quantitative polymerase chain reaction (qPCR). Results: RC significantly altered the expression levels of 5,316 genes in A549 cells and 7,845 genes in H1650 cells. The most up-regulated genes were C11orf96 and RMRP, while the most down-regulated genes were ZBTB6, NUFIP1 and PTPN20. GSEA results showed that down-regulated genes were mainly related to cell cycle, DNA replication, RNA degradation and splicing pathways. KEGG analysis showed that down-regulated genes were enriched in cell cycle, spliceosome, and nucleocytoplasmic transport pathways. GO analysis showed that DEGs mainly involves in DNA replication, chromosome separation and non-coding RNA processing. PPI network analysis revealed hub genes, and qPCR confirmed that RC inhibits their expression levels. Conclusions: RC inhibits the proliferation of A549 and H1650 cells via multiple signaling pathways and targets, where cell cycle-related genes, including CDK1, CDK2, CCNA2, CCNB1 and CDC6, are the core targets.

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