Chemical Constituents of the Alpine Bistort Rhizome and Network Pharmacology Study on Its Anti-diarrheal Effects
DOI:
https://doi.org/10.62767/jecacm701.1819Keywords:
alpine bistort rhizome, chemical composition, diarrhea, network pharmacology, molecular dockingAbstract
To systematically analyze the antidiarrheal active components and potential mechanisms in the rhizome of alpine bistort (Bistorta vivipara (L.) Gray). This study separated and identified 9 categories of chemical components in the alpine bistort rhizome employing methods such as high-performance liquid chromatography (HPLC), high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD), high-performance liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), inductively coupled plasma mass spectrometry (ICP-MS), etc. A total of 126 chemical components were identified from the samples of alpine bistort rhizome, including 4 polysaccharides, 6 monosaccharides that compose polysaccharides, 3 free sugars, 17 amino acids, 3 nucleosides, 2 sterols, 14 organic acids, 21 flavonoids, 2 glycosides, 1 Sugar-conjugated polymer, 34 volatile components, and 19 elements.The main chemical components, targets, and potential signaling pathways of alpine bistort rhizome for preventing diarrhea were screened using network pharmacology methods, and molecular docking was performed on key compounds and targets. The results showed that 50 active components in alpine bistort rhizome could regulate the Tumor Necrosis Factor (TNF) signaling pathway, lipid and atherosclerosis , Phosphoinositide 3-Kinase-Protein Kinase B (PI3K-Akt) signaling pathway and Hypoxia-Inducible Factor-1 (HIF-1) signaling pathway by acting on 227 target points, thus exerting the anti-diarrhea effect. The molecular docking results revealed that the core targets Signal Transducer and Activator of Transcription 3 (STAT3), Epidermal Growth Factor Receptor (EGFR), TNF, Heat shock protein 90kDa alpha (cytosolic), class A member 1 (HSP90AA1), and Recombinant Caspase 3 (CASP3) exhibited good binding ability with the active compounds.This study preliminarily elucidated the pharmacological basis of the rhizome of alpine bistort and its potential mechanism, providing a scientific foundation for its clinical application and quality control.
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