MiR-138-5p Targets Nir1 to Regulate Glioma Cell Invasion

Pingping Chen; Yong Ping; Yino Zhao; Fenhua Liang; Baihui Song; Ling Wang; Huiling Li; Shuyan Sun

Authors

Pingping Chen Department of Pathology, Rizhao People's Hospital, 276827 Rizhao, Shandong, China Author
Yong Ping Rizhao Central Hospital, 276899 Rizhao, Shandong, China Author
Yino Zhao Department of Pathology, Rizhao People's Hospital, 276827 Rizhao, Shandong, China Author
Fenhua Liang Department of Pathology, Rizhao People's Hospital, 276827 Rizhao, Shandong, China Author
Baihui Song Rizhao Central Hospital, 276899 Rizhao, Shandong, China Author
Ling Wang Department of Pathology, Rizhao People's Hospital, 276827 Rizhao, Shandong, China Author
Huiling Li Department of Pathology, Rizhao People's Hospital, 276827 Rizhao, Shandong, China Author
Shuyan Sun Department of Pathology, Rizhao People's Hospital, 276827 Rizhao, Shandong, China Author

Keywords:

Glioma, invasion, MiR-138-5p, Nir1

Abstract

Objective: To investigate the relationship between miR-138-5p and PYK2 N-terminal domain-interacting receptor 1 (Nir1) and determine whether miR-138-5p affects glioma invasion through binding with Nir1. Methods: The expression level of miR-138-5p in different glioma cell lines and its effect on Nir1 protein in glioma cells U251 and H4 were compared. The invasion ability of glioma cells after transfection, and whether miR-138-5p could target Nir1 were detected. Results: The level of miR-138-5p in low-invasive human glioma cells H4 was significantly higher than that in highly invasive human glioma cells U251, LN229 and U87 (p < 0.05). In U251 and H4 cells, the expression of Nir1 protein was lowered in the miR-138-5p overexpression group compared to the miR-negative control (miR-NC) group, but increased in miR-138-5p inhibitor group relative to inhibitor NC group. However, Nir1 mRNA level in miR-138-5p overexpression group had no significant changes compared with the miR-NC group (p > 0.05). The number of U251 and H4 cells in the miR-138-5p overexpression group passing through the small hole in the basement membrane was significantly less than that in the miR-NC group (p < 0.05). MiR-138-5p overexpression evidently reduced luciferase activity of Nir1-3'-UTR. Conclusion: MiR-138-5p binding to Nir1 in glioma cells can inhibit the invasion of glioma cells by reducing the translation of Nir1 and the expression of Nir1 protein.

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